Data underlying the publication "Assembly and cell-free expression of a partial genome for the synthetic cell"
Wat erin zit
*** Assembly and cell-free expression of a partial genome for the synthetic cell *** Authors: Céline Cleij, Laura Sierra Heras, Ellen Zwiers, Pascale Daran-Lapujade, Christophe DanelonDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology;Department of Biotechnology, Delft University of Technology;Toulouse Biotechnology Institute (TBI), Université de Toulouse, CNRS, INRAE, INSA Corresponding authors: Pascale Daran-Lapujade and Christophe DanelonContact information: p.a.s.daran-lapujade@tudelft.nl and danelon@insa-toulouse.fr *** General introduction ***This dataset contains data collected during experiments for the manuscript "Assembly and cell-free expression of a partial genome for the synthetic cell". Data was collected in 2021-2025. *** Methodological information ***Supplementary data 1-4 were prepared in Excel. The overview of relevant mutations in Supplementary data 4.5 is based on mutations in consensus sequences and raw reads obtained from sequencing.Supplementary data 5: Designed MSG sequences (GenBank) were prepared with the SnapGene software, using the plasmid maps of the sequenced template plasmids and the designed primer …
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