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Sample dataset and software for FAST-EM array tomography

4TU.ResearchDatavia 4TU.ResearchData (via DataCite) · bijgewerkt 15 juli 2024

Wat erin zit

This repository contains a sample electron microscopy dataset produced with "FAST-EM array tomography" [1] and accompanying software to produce a 3D reconstruction of this dataset. FAST-EM array tomography is a technique for acquiring and reconstructing volume (3D) multibeam electron microscopy datasets. The sample dataset is part of a larger volume (3D) electron microscopy dataset included in the publication Kievits et al. (2024). This sample dataset is used for (automatic) testing of the software presented in the publication and as a demonstration example of 3D reconstruction. Datasets MCF-7 cells 3 serial sections of cultured Michigan Cancer Foundation-7 (MCF-7) cells (partial dataset). The dataset contains 25 images per section in a 5 x 5 grid, covering an area of 120 x 120 microns in each section of the sample. The `megafield_field_meta_data.yaml` contains metadata from the acquisition and is used in the processing. Full dataset visualization Partial dataset link: https://webknossos.tnw.tudelft.nl/links/BIduioDOrpiBh_EDFull dataset link: https://webknossos.tnw.tudelft.nl/links/9TwKDPdjNa4JHIYt Organization ┌ 20231107_MCF7_UAC_test (project)├──┬ S001 (section --> z)│ ├── …

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