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Microscopy data, code and analysis underlying the publication "Optical STEM detection for scanning electron microscopy"

4TU.ResearchDatavia 4TU.ResearchData (via DataCite) · updated 31 October 2023

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Raw microscopy data underlying the publication, structured per experiment (figure). The imaging setting that is varied in the experiments (exposure/dwell time, beam current, landing energy) is indicated by the folder or image name. The images may have a data bar indicating the relevant acquisition settings.For more information on the sample preparation and image acquisition, see the Methodology section in the publication.The code and data analysis notebooks are included in the accompanying Github repository: https://github.com/hoogenboom-group/Kievits-OSTEM-2023 Data set structure: 1_Optimization-OSTEM : contains the images for the landing energy optimization (Figure 2 in the article). Images have a pixel size of 1 nm and where acquired with increments of 500 eV (electron volts). Dwell times are indicated in the folder names. The 'Qualitative' folder contains a subset of these data for ease of plotting Figure 2A. 2_Qualitative-comparison : contains qualitative images of rat pancreas and zebrafish larva (Figure 3). Both raw images (with metadata in TIFF header) and cropped images are included (used for publication). Images have a pixel size of 4 nm. 3_SNR-comparison-detectors: …

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