Data supporting the analysis of sequencing data for identification of lactic acid bacteria (LAB) and yeast species in traditionally fermented maize ogi, part of the research " Traditional maize ogi production and the assessment of selected species for the development of a starter culture "
Wat erin zit
Raw data related to Sanger sequencing of the genomic DNA of 105 LAB and 71 yeasts isolated from traditionally fermented maize starch called “ ogi ” in Benin, and results of the sequence alignments after blasting using the NCBI Nucleotide collection (nt) database. The sequences and sequence alignment results are labelled “B1” to “B105” for LAB, and “Y1” to “Y71” for yeasts. Samples of maize starch slurry were obtained from traditional processors in five municipalities in Southern Benin. LAB and yeasts were isolated after 6 - 24 h of fermentation of the maize starch slurry. Sanger sequencing was performed via the TubeSeq service of Eurofins Genomics (the Netherlands) after colony-PCR implemented using PCRBIO HS VeriFi™ Polymerase to amplify the regions V3/V4 of the 16S rRNA encoding genes for the LAB isolates, and the region ITS1 rRNA for the yeast isolates. The section of the sequences containing “A”, “G”, “C” and “T” nucleotides were copied, avoiding non-templated nucleotides (“N”) as much as possible. The best fitting scientific name for the sequences producing significant alignments with a percentage of identification above 90% was selected.
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